VI SEMESTER BSC Biochemistry

VI SEMESTER

Paper 6.1; Biochemistry-VII

1. Introduction to metabolism

2h

Anabolism, catabolism, stages in catabolism, compartmentalization of metabolic pathways in cells and

energy conversation.

2. Carbohydrates metabolism

2h

Glycolysis entry of other carbohydrates into glycolysis, the fate of pyruvate, the citric acids cycle, the

energetics of glucose metabolism. Enzymatic control of glycolysis and TCA cycle. Outline of pentose

phosphate pathway and its significance. The cory cycle, outline of gluconcogenesis and glycogen

metabolism. The amphipathic and integrating role of the citric acids cycle. Substrate level

phoshorylation, regulation of blood sugar level. Diabetes mellitus. Glycogen storage disorder and Cori

disease.

3. Lipid Metabolism     9 h

14

The b-oxidation pathway even and odd numbers, saturated and unsaturated fatty acids. Structure and

function of fatty acyl synthase. Energetics of total oxidation. General scheme of biosynthesis of fatty

acids,. Outline of cholesterol biosynthesis. Ketone bodies, atherosclerosis.

3. Amino acidsmetabolism

9h

General reaction of amio acids metabolism- transamination, deamination and decarboxilation. The

Urea cycle and its regulation. Biosynthesis of glycogen, alanin cysteine and aspartic acid.

Biosynthesis of biologically active amines-epinephrine.

Non- epinephrine. Histomine and poly amines. Disorders of aminoacid metabolism PKU and AKU.

5. Nucleic Acid metabolism

4h

Biosynthesis and regulation of purine and pyrimidine nucleotides. Metabolic disorders. Orotic acid

urea and Gout.

6. Photosynthesis

4h

The photosynthesis apparatus and pigments. Outlines of lights and dark reactions C3 and C4 plants.

VI SEMESTER BIOCHEMISTRY PRACTICAL-VII

1. Colorimetric estimations;

a) Protein by F-C method,

b) Iron using ano

c) Phosphate by Firke

d) Creatinne in urine

e) Blood sugar by Folic method

f) Estimation of lactose in milk

2. Determination of the equivalent conductivity of an electrolye.

3. Conductomtric titration of an amino acid again hydrochloric acid and sodium.

VI BIOCHEMISTRY PRACTICAL

Enzymes

1. Preparation of buffer and determination of pH (acetate-pH5.0; phosphate pH8.0)

2. Determination of the amylase activity in salivn of Effect of time on amylase activity.

3. Effect of temperature and pH on the activity of the salivary amylase.

4. Assay of phosphates from potato/green gram.

5. Qualitative analysis of body fluids such as blood (urea, total count, plasma volume). And urine

(pH, odor, colour, sp. Gravity, proteins, blood, sugar)

6. Estimation of glucose by Hagedorn-Jenson method.

7. Determination of acidity of urine.

15

Paper 6.2; Biochemistry –VIII

1. Miroscopy

2h

the compound microscope and its uses. Brief mention on electron microscope and

applications.

2. Classification of micro organizations

2h

Eubactera, archeobactera, fungi, protozoa and viruses

3. Bacterial cell structure

6h

Ultra structure and composition of cell wall, chromosomes, plasmid, ribosomes, cell

membrane, flagella (Motility) capsule and spores. Gram-stain classification of bacteria as Gram

positive and Gram negative, Spore stains.

4. Viruses

5h

Brief account of animal viruses polio, influenza, HIV; plant viruses –TMV and bicteriophage

Teven (Mature and infected cycle)

5. Cultivation of micro organizations

4 h

Nutritional requirements, temperature, pH and oxygen consideration for optimal growth curve,

control of microorganism by chemical and physical method.

6 Microbial technology

3h

Use of microorganism in- a) fermentation – production of ethanol baker’s yeast and penicillin, b)

biodegradation of sewage, c) Single cell protein-spirulina.

7. Immunology

10h

Innate and acquired immunity, antigen, antigenic determinants and heptenes, T and B-limphocytes,

cellular and humeral immunity, immune globulin-classes, and function, structure of IgG, Antigenantibody

reaction, allergy, vaccines, ABO blood group, blood typing.

8. Introduction to Recombinant DNA Technology

16

8h

Enzymes used in recombined DNA technology; Vectors, chimeric DNA molecules, introduction of

recombined DNA molecules in host cells. Selection of transformed cells. Libraries and library

construction. Probes, blotting and hybridization techniques. Application of recombined DNA

technology. Insulin production.

BIOCHEMISTRY PRACTICAL-VIII

1. Washing and sterilization of glassware.

2. Preparation of media-plates, stab, slant, 

REFERENCE:

DNA Replication inprokaryotic organism

DNA Replication inEukaryotic organism

Genetic engineering andcloning

Human genetics videonotes

I Semesterbiotechnology(cell biology and genetic)

II semesterbiotechnology(Microbiology and biostatistic)

III Semesterbiotechnology (Biochemistry and biostatistic)

IV Semesterbiotechnology (molecular biology)

V Semesterbiotechnology (paper5-genetic

VI Semesterbiotechnology(plant biotechnology and industrial biotechnology)

Genetics

I semesterGenetics(fundamental of cell biology)

II semester genetics(principles of Genetic)

III semester genetics

IV semester genetics(molecular Genetics)

V semester genetics(paper5 Recombinant DNA technology)

Paper 6 Human Genetics

Vi semester Genetics(paper7 Advance Genetic)

Paper8 Genetics (Applied Genetics)

   

    BIOCHEMISTRY

I semester biochemistry

II semesterbiochemistry

III semesterbiochemistry

Iv semesterbiochemistry

V semester biochemistry

Vi semesterbiochemistry