III semester Biochemistry

III SEMESTER

Paqper3.1; Biochemistry-III

1. Colloids     2h

Types and methods of preparation and purification sob. Electrical properties of colloids. Tyndall effect.

Ultra microscope. Brownian movement. Emulsions and emulsifiers Gel. Application of emulsions of in

lipid chemistry.

2.Enviormental Chemistry  5h

Air population and their control-carbon dioxide, sulphur dioxide, oxides of nitrogen and hydrocarbons.

Carbon dioxide and the green home effect. Chlorofluorocarbons and the ozone layers. Biochemical

toxicity and detoxification of chromium, leads, mercury, cadmium and arsenic. Water pollution Bod

and COD. Treatment of sewage and industrial effluent.( tanning and electroplating). Pesticide hazards.

Disposal of radioactive wastes.

3. Coordination Chemistry

4h

Transition metal ions and oxidation stages. Double and complex salt. Werner’s and valence bond

theories. Geometrical optical isomerism.

4. Bioinorganic Chemistry

4h

Metal ions in biological system. Roles of Iron in myoglobin and cytochrome. Copper in haeinocyanin.

Magnesium in chlorophyil, Cobalt in vitamin B12 and Molybdennrn. Metallo-enzymee.

5. Photochemistry    3h

Laws of photochemistry, chemihmuinescence biolumiuescence, photocatalysis and photochemical

reaction.

6. Identification and separation techniques  11h

Spectroscope: Application of UV-visible and IR spectroscope in the identification of common

functional groups. Chromatography: General principal. Adsorption –TLC, partition, paper, GLC.

Electrophoresis Principal. Paper and seal electrophoresis. Ultra centrifugation: Principal.

Sedimentation Method of crystallization and x-ray diffraction-proteins and nucleic acids. NMR and application on Biochemistry.

7. Stereochemistry

6h

Stereoisomerism: Types Stereo chemical technology. Optical isomerism: Molecular dissymmetry

Chirality glyceraldehydes, lactic and tartaric acid. Nomenclature of enantiomers-The KS system DL

Rotation, racemisation and resolution. Fischer projection formula. Geometrical isemerism: Cis trans

isomerism in alkenes and ring compounds. Structure and properties of malice and firmaric acid(E)-

(Z)system of specifying geometrical isommer. Significance of chirality in the biological world.

8. Carboxylic Acids    3h

Meaning and usefulness of pK values. Acidity of monocarboxylic acid strength. Hydroxy acid:

Preparation and properties of lactic acid. Structure of tartaric malic, citric and isocitric acids.

Dicarboxylic acids. Saturated dicarboxylic acids-effect of heat on the first five members.Kito acids

Structures of pyruvic, "-ketoglutric acids. Properties of pyruvic acid.

9. Amines     2h

Classification. Isomerism. Distinguishing reaction of 10, 20 and 30 amines. pKb values of amines. Effect

of substituents on the basic strength of amines. Some biologically important amines.

10. Heterocylic Compounds.

2h

Structure formulae and occurrence of furan, pyran, thiophene, thizole, pyrrole, imidazole, pyridine,

pyrimidine, purine, isoallxazine and indol. Reaction of imidazole and pyridine. Aromaticity of furan,

thiophene, Pyrrol and Pyridine.

11. Terpense    4h

Isoprene rule. Classification with examples, biologycal Importance and occurrence of the following:

liomonene, menthol, santoin, juvenil hormone I, abscisin II, phytol, gibberlic acid, lanosterol,

lycopene, polyphenols-dolichols, Steroids, basic ring system. Structure of cholesterol, steoid hormones

(testosterone and esradiol,). Structure and importance of $-carotene, chlorophyll a, ubiquinone and

plastoquinone.

12. Alkalolds   3h

Introduction and general characteristics. General method of extraction. Structure and medicinal uses

of nicotine, atropine and LBD. Phyocemicals- Introduction and uses.

13. Drugs     2h

Chemotherapy. Classification of drug. Synthesis and uses of sulfanilamide, paludrine and pentothal.

14. Antibiotics  2h

Definition: Types Sources, structures and antimicrobial spectrum of action of penicillin,

chloroamphenicol, streptomycin and tetracyclines.

15. Pesticides  2h

Structure and uses of the following insecticides DDT, gammexane lindane allethrin and malathion.

Berbicides: 2, 4-D and 2, 4, 5T.

III SEMESTER BIOCHEMISTRY PRACTICAL

1. Systematic qualitative analysis of sample organic compound – alcohol, phenols, carboxylic

acids, aldehydee, ketones, esters, aromatic hydrocarbons, halo-compounds, metro-compounds,

aminsues and amider.

2. Detection of adulterants .

Reference:

DNA Replication inprokaryotic organism

DNA Replication inEukaryotic organism

Genetic engineering andcloning

Human genetics videonotes

I Semesterbiotechnology(cell biology and genetic)

II semesterbiotechnology(Microbiology and biostatistic)

III Semesterbiotechnology (Biochemistry and biostatistic)

IV Semesterbiotechnology (molecular biology)

V Semesterbiotechnology (paper5-genetic

VI Semesterbiotechnology(plant biotechnology and industrial biotechnology)

Genetics

I semesterGenetics(fundamental of cell biology)

II semester genetics(principles of Genetic)

III semester genetics

IV semester genetics(molecular Genetics)

V semester genetics(paper5 Recombinant DNA technology)

Paper 6 Human Genetics

Vi semester Genetics(paper7 Advance Genetic)

Paper8 Genetics (Applied Genetics)

   

    BIOCHEMISTRY

I semester biochemistry

II semesterbiochemistry

III semesterbiochemistry

Iv semesterbiochemistry

V semester biochemistry

Vi semesterbiochemistry